Moleküler Biyoloji ve Genetik Bölümü / Department of Molecular Biology and Genetics
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Publication 24-Epibrassinolide Induces SSAT and Causes Caspase-Dependent Apoptosis In Different Prostate Cancer Cell Lines(2011) Bolkent, Şehnaz; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 156421; 113920; 3894; 6125Publication An investigation on regulation on trichome metabolism related genes against salt stress in soybean (Glycine max L. Merr.)(2014-06-25) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Antioxidative defense system differences to drought stress of tomato cultivars(2014) AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 185510; 113987; 6653Publication Autocrine Growth Hormone-Triggered Curcumin Resistance Abolished by NF-κB Signaling Pathway Dependent on Inflammatory Cytokines and Active Polyamine Catabolic Machinery in MCF-7, MDA-MB-453 and MDA-MB-231 Breast Cancer Cells(2017) Çoker Gürkan, Ajda; Çelik, Merve; Uğur, Merve; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 125860; 113920; 156421; 6125Autocrine Growth Hormone (GH) induces cell growth, proliferation metastasis in breast cancer. Curcumin is a promising therapeutic agent in cancer through affecting different molecular targets. Our aim was to demonstrate the molecular machinery of curcumin-mediated apoptosis in autocrine GH + MCF-7, MDA-MB-453 and MDA-MB-231 breast cancer cells (BCCs). Stable GH expressing BCCs were generated by GH gene insert PC3.1 plasmid transfection and Neomycin selection. Although GH + cells are resistant to curcumin treatment, dose-dependent drug exposure decreased cell viability, inhibited colony formation, invasion-metastasis via suppressing GH expression in each BCCs. Anti-hormonal concentration of curcumin (20 µM for MCF-7, MDA-MB-453 and 25 µM for MDA-MB-231) inhibited NF-κB p65 (Ser 536) phosphorylation and decreased DNA binding activity of NF-κB p65 in autocrine GH expressing BCCs. In addition, autocrine GH-mediated IL-1α, IL-6, IL-1β pro-inflammatory cytokine expressions downregulated by curcumin treatment. Moreover, curcumin overcome autocrine GH triggered drug resistant and induced caspase-mediated apoptotic cell death through activating Polyamine (PA) catabolic pathway enzymes which led to generation of toxic by-products such as H2O2 in MCF-7, MDA-MB-453 and MDA-MB-231 GH + BCCs. In conclusion, curcumin could overcome GH-mediated resistant phenotype via modulating NF-κB-mediated inflammatory cytokine expression and PA catabolic machinery activation in breast cancer cells.Publication Celastrol Modulates Lipid Synthesis via PI3K/Akt/mTOR Signaling Axis to finalize Cell Death Response in Prostate Cancer Cells(2017) Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; RENCÜZOĞULLARI, ÖZGE; 113920; 222563; 125860; 156421; 6125FASN is key enzyme during lipid biogenesis is associated with prostate cancer. In this study, we aim to investigate the potential role of celastrol, root extracts of Tripterygium wilfordii on modulation of lipid biosynthesis-associated PI3K/Akt signaling. To determine the effect of celastrol on cell viability, prostate cancer cells were exposed with celastrol in dose dependent manner. AR (+) LNCaP and AR (−) DU145 and PC3 cell viability were inhibited by celastrol with IC50 in the range of 0.05–1 µM. To address the role of celastrol on cell death mechanism, celastrol-treated prostate cancer cells were evaluated with immunoblotting and flow cytometric analysis. Celastrol significantly upregulated PARP and caspase 9 cleavage also increased sub-G1 population. Celastrol also inhibited cell migration and invasion. These effects were associated with decreased PI3K/Akt signaling axis and downregulation of epithelial mesenchymal transition in prostate cancer cells. Likewise, lipid biosynthesis was downregulated with celastrol, however inhibition of PI3K/Akt signaling axis via LY294002 further decrease the cell migration and proliferation rate in prostate cancer cells. Our data suggest that, celastrol suppressed cell proliferation via inhibition of lipid biosynthesis through downregulation of PI3K/Akt signal axis. Targeting lipid metabolism-related enzymes in prostate cancer may offer new avenues for therapeutic approaches.Publication Combined Treatment of Roscovitine and DFMO Induced Caspase Dependent Apoptosis in MCF-7 cells. 2nd(2010) Ünsal, Zeynep Narçin; Çoker Gürkan, Ajda; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 156421; 113920; 6125; 125860Publication Comparison of the effect of different cytokinins on chlorophyll, protein content and peroxidase activity(2001) Ünsal, Zeynep Narçin; Çağ, Serap; ARISAN, ELİF DAMLA; 6125; 113920Publication Cox-2 inhibitor ibuprofen induced apoptosis via altering different signalling routes in HT-29 and DLD-1 colon cancer cells(2015) Yalçın, Gizem Damla; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 113920; 125860; 156421; 6125Publication Çukurovada Yetiştirilen Calland ve Amsoy 71 Soya Çeşitlerinde Verim ve Miktarının Nükleer Teknikle Geliştirilmesi(1986) Özbek, N.; Atilla, S.; Sağel, Z.; ATAK, ÇİMEN; 6653Publication Curcumin induced-apoptotic cell death in GH overexpressed MDA-MB-231 breast cancer in time dependent manner(2015) Çelik, Merve; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 125860; 113920; 156421; 6125Publication Cyclin Dependent Kinase Inhibitors Modulates Polyamine Metabolism in MDA-MB-231 Cells(2010) Ünsal, Zeynep Narçin; Çoker Gürkan, Ajda; Özdemir, İpek; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 6125; 113920; 156421; 125860Publication Cytokine network is critical in growth hormone-induced resistance mechanism against curcumin which modulates JAK/STAT/SOCS pathway in MDA-MB-231 and MCF-7 breast cancer cells(2016) Çelik, Merve; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 125860; 113920; 156421; 6125Publication Development of Superoxide Dismutase Loaded Microspheres(2007-03-27) Akbuğa, Fatma Jülide; ÇELİK, ÖZGE; 113987; 11241Publication Diethylnorspermine (DENSpm) Induced Apoptosis in SW480 Colon Cancer Cells(2012) İlhan, Halime; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 125860; 113920; 156421; 6125Publication Differences in Proline Accumulation and Gene Expression Profiles of Salt-Tolerant Soybean Mutants(Springer, 233 Spring Street, New York, Ny 10013, United States, 2012-08) Ünsal, Selin Gül; ÇELİK, ÖZGE; 113987Publication Differential regulation of antioxidative gene expressions in response to salt stress in rice(International Congress on Transcriptomics 27-29 Temmuz 2015, 2015) Candar, Bilgin; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 195745; 6653Rice (Oryza sativa L.) is one of the most important crops because it is a nutritional source of more than one-third of the world population. Soil salinity is one of the most important abiotic stress factors that affect plant growth and productivity adversely. Rice growth and yield is also affected by salinity and at the seedling stage although it is known to be susceptible to salinity. Reactive oxygen species are induced by salt stress and some responsive mechanisms are evolved against to the detrimental effects caused by salt stress. Salinity response is especially controlled by obtaining homeostasis between antioxidative mechanisms and accumulation of reactive oxygen species (ROS) produced as a result of oxidative stress caused by salinity. It is known that combined expression profiles of antioxidative system enzymes may provide increased tolerance capacity. Therefore, the expression profiles of antioxidative enzymes in two different rice cultivars which have different salt sensitivities at four different salt stress conditions were determined. Salinity responses of two different rice varieties were investigated at seedling stage. Four different salinity treatments were then applied using Yoshida solution containing 0, 30, 90, 150 and 210 mM NaCl to the nutrient solution for seven days. At the end of the seven days, the leaves were harvested and stored at -20oC for further experiments. The salt stress responsive gene specific primers were amplified by designed primers due to the 3?-UTR regions of each of the following genes by aligning all available related genes in the databases of NCBI and KOME: Mn-SOD, Cu/Zn-SOD, Fe-SOD, Cytosolic APX, Thylakoid-bound APX, stromal APX, Cytosolic GR and CatA. Mn-SOD was consisted with the trend of variation in SOD activities of rice varieties. The expression patternof CAT A gene was markedly decreased compared to control in both rice varieties. Str-APX gene expression was up-regulated during salt stress treatments in both rice varieties. Transcript levels of Cyt-APX and Thy-APX were up-regulated in accordance with increasing salt stress in Osmanc?k-97 variety. The expression pattern of gene encoding enzyme Cyt-GR1 showed a gradual up-regulation as a response to subjected increasing NaCl stress in Mevl�tbey variety while only after 90 mM treatment, an up-regulation was observed for Osmanc?k variety. These data indicated that the antioxidative responses of salt tolerant and salt sensitive rice varieties are differentially regulated.Publication Diminished Cyclin Dependent Kinase Activity and mTOR Are Critical In The Cell Death DecisionThrough Affecting STAT Signalling Differently In Prostate Cancer Cells(2017) Gümüşkaptan, Çağrı; Berrak, Özge; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 113920; 274299; 156421; 125860; 6125Publication EBR induced autophagy in colon carcinoma cell lines(2015-07) Adacan, Kaan; Çoker Gürkan, Ajda; Ünsal, Zeynep Narçin; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN; 156421; 113920; 125860; 6125Publication Effect of magnetic field on Paulownia seeds(2000) Yurttaş, B.; Alikamanoğlu, Sema; Topçul, F.; Rzakoulieva, Aytekin; Danilov, V.; ATAK, ÇİMEN; 6653; 176641Publication Effects of Salinity Stress on Densities of Trichomes, Glandular Trichomes, and Stomata in Two Soybean Varieties(Springer, 233 Spring St, New York, Ny 10013 USA, 2010-09) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 4019; 6653