Browsing by Author "Ergin, Sevgi"

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    Molecular Identification of Listeria monocytogenes and Escherichia coli O157: H7 Isolated from Fresh Kashar Cheese and Milk Creme
    (2019-01-07) Dümen, Emek; Bayrakal, Gülay Merve; Ergin, Sevgi; EKİCİ, GÖZDE; 110385; 218728
    In this study, it is aimed to analyze the risk factors related to Listeria monocytogenes and Esherichia coli O157:H7 monitoring in fresh kashar cheese and milk creme in the province of Istanbul. It has 100 pieces of fresh kashar cheese and 100 pieces of milk creme material. Samples were confirmed by PCR methods and genetic basis with conventional microbiological sowing methods and analysis methods. Another case for the study is to obtain significant epidemiological environment for the field strains in the country in terms of L. monocytogenes and E. coli OH157: H7, where high polymorphic protein structures and rapid genetic variation exist. In the study, 4 (2%) of E. coli OH157: H7 and 6 (3%) of L. monocytogenes were found among 200 milk slices and fresh kashar cheese samples. Those microorganisms can easily reproduce in natural environment such as soil and silage and with primer/secondary contamination, they appear to be health threatening food related pathogens. It is suggested to have studies for optimal food safety and this can be provided in accordance with appropriate legislation.
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    Presence of Foodborne Pathogens in Seafood and Risk Ranking for Pathogens
    (Mary Ann Liebert, Inc., 2020) Dümen, Emek; EKİCİ, GÖZDE; Ergin, Sevgi; Bayrakal, Gülay Merve
    This study aims at examining the contamination of coliform bacteria, Escherichia coli, Listeria monocytogenes, Vibrio vulnificus, and Vibrio cholerae, which carry extremely serious risks to the consumer health, in 700 seafood belonging to 4 different (raw sea fish, raw mussels, raw shrimp, and raw squid) categories. The total number of samples was determined as 700. When the obtained results were viewed in total, they were found to be 48.14%, 18.71%, 8.57%, and 3.42% for coliform bacteria, E. coli, L. monocytogenes, and V. vulnificus, respectively. V. cholerae, one of the factors studied, was not found. Conventional microbiological cultivation methods were used in the analysis stage as well as the real-time PCR method. This study aims at making a risk ranking modeling for consumer health based on product category and pathogens by interpreting the results of the analysis with statistical methods. According to the statistical analysis, significantly binary correlations were determined among some parameters that stimulate one another for reproducing. In the light of the obtained results of the study, it has been concluded that the studies of the most detailed examinations of the microbiological risks associated with seafood, forms of microbial pollution and microorganisms that cause deterioration in seafood and threaten consumer health and the path that their epidemiologies follow, are of primary importance to both protecting consumer health and obtaining safe and quality seafood.