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dc.contributorFen Edebiyat Fakültesi / Faculty of Letters and Sciences Moleküler Biyoloji ve Genetik / Molecular Biology and Geneticstr_TR
dc.contributor.authorArısan, Elif Damla
dc.contributor.authorRencüzoğulları, Özge
dc.contributor.authorÇoker Gürkan, Ajda
dc.contributor.authorObakan Yerlikaya, Pınar
dc.contributor.authorÜnsal, Zeynep Narçin
dc.date.accessioned2019-02-04T13:07:24Z
dc.date.available2019-02-04T13:07:24Z
dc.date.issued2017
dc.identifier.issn2504-3900
dc.identifier.urihttps://doi.org/10.3390/proceedings1100992
dc.identifier.urihttps://hdl.handle.net/11413/4409
dc.description.abstractFASN is key enzyme during lipid biogenesis is associated with prostate cancer. In this study, we aim to investigate the potential role of celastrol, root extracts of Tripterygium wilfordii on modulation of lipid biosynthesis-associated PI3K/Akt signaling. To determine the effect of celastrol on cell viability, prostate cancer cells were exposed with celastrol in dose dependent manner. AR (+) LNCaP and AR (−) DU145 and PC3 cell viability were inhibited by celastrol with IC50 in the range of 0.05–1 µM. To address the role of celastrol on cell death mechanism, celastrol-treated prostate cancer cells were evaluated with immunoblotting and flow cytometric analysis. Celastrol significantly upregulated PARP and caspase 9 cleavage also increased sub-G1 population. Celastrol also inhibited cell migration and invasion. These effects were associated with decreased PI3K/Akt signaling axis and downregulation of epithelial mesenchymal transition in prostate cancer cells. Likewise, lipid biosynthesis was downregulated with celastrol, however inhibition of PI3K/Akt signaling axis via LY294002 further decrease the cell migration and proliferation rate in prostate cancer cells. Our data suggest that, celastrol suppressed cell proliferation via inhibition of lipid biosynthesis through downregulation of PI3K/Akt signal axis. Targeting lipid metabolism-related enzymes in prostate cancer may offer new avenues for therapeutic approaches.tr_TR
dc.language.isoen_UStr_TR
dc.relation2nd International Conference on Natural Products forCancer Prevention and Therapytr_TR
dc.subjectcelastroltr_TR
dc.subjectFASNtr_TR
dc.subjectprostate cancertr_TR
dc.titleCelastrol Modulates Lipid Synthesis via PI3K/Akt/mTOR Signaling Axis to finalize Cell Death Response in Prostate Cancer Cellstr_TR
dc.typeArticletr_TR
dc.contributor.authorID113920tr_TR
dc.contributor.authorID222563tr_TR
dc.contributor.authorID125860tr_TR
dc.contributor.authorID156421tr_TR
dc.contributor.authorID6125tr_TR


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