Publication:
Gene Editing and RNAi Approaches for COVID-19 Diagnostics and Therapeutics

dc.contributor.authorBerber, Burak
dc.contributor.authorAydın, Cihan
dc.contributor.authorKocabaş, Fatih
dc.contributor.authorGüney-Esken, Gülen
dc.contributor.authorYılancıoğlu, Kaan
dc.contributor.authorKaradağ-Alpaslan, Medine
dc.contributor.authorÇalıseki, Mehmet
dc.contributor.authorYüce, Melek
dc.contributor.authorDemir, Sevda
dc.contributor.authorTaştan, Cihan
dc.date.accessioned2022-11-18T08:25:07Z
dc.date.available2022-11-18T08:25:07Z
dc.date.issued2021
dc.description.abstractThe novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way.en
dc.identifier28
dc.identifier.citationBerber, B., Aydin, C., Kocabas, F., Guney-Esken, G., Yilancioglu, K., Karadag-Alpaslan, M., ... & Tastan, C. (2021). Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics. Gene therapy, 28(6), 290-305.
dc.identifier.issn0969-7128
dc.identifier.pubmed33318646
dc.identifier.scopus2-s2.0-85105424610
dc.identifier.urihttps://doi.org/10.1038/s41434-020-00209-7
dc.identifier.urihttps://hdl.handle.net/11413/7944
dc.identifier.wos000598702600001
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.journalGene Therapy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSARS-CoV-2
dc.subjectTherapy
dc.subjectRibozymes
dc.titleGene Editing and RNAi Approaches for COVID-19 Diagnostics and Therapeuticsen
dc.typeReview
dspace.entity.typePublication
local.indexed.atwos
local.indexed.atpubmed
local.indexed.atscopus
local.journal.endpage305
local.journal.issue6
local.journal.startpage290

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