Publication: Gene Editing and RNAi Approaches for COVID-19 Diagnostics and Therapeutics
dc.contributor.author | Berber, Burak | |
dc.contributor.author | Aydın, Cihan | |
dc.contributor.author | Kocabaş, Fatih | |
dc.contributor.author | Güney-Esken, Gülen | |
dc.contributor.author | Yılancıoğlu, Kaan | |
dc.contributor.author | Karadağ-Alpaslan, Medine | |
dc.contributor.author | Çalıseki, Mehmet | |
dc.contributor.author | Yüce, Melek | |
dc.contributor.author | Demir, Sevda | |
dc.contributor.author | Taştan, Cihan | |
dc.date.accessioned | 2022-11-18T08:25:07Z | |
dc.date.available | 2022-11-18T08:25:07Z | |
dc.date.issued | 2021 | |
dc.description.abstract | The novel coronavirus pneumonia (COVID-19) is a highly infectious acute respiratory disease caused by Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV-2) (Prec Clin Med 2020;3:9-13, Lancet 2020;395:497-506, N. Engl J Med 2020a;382:1199-207, Nature 2020;579:270-3). SARS-CoV-2 surveillance is essential to controlling widespread transmission. However, there are several challenges associated with the diagnostic of the COVID-19 during the current outbreak (Liu and Li (2019), Nature 2020;579:265-9, N. Engl J Med 2020;382:727-33). Firstly, the high number of cases overwhelms diagnostic test capacity and proposes the need for a rapid solution for sample processing (Science 2018;360:444-8). Secondly, SARS-CoV-2 is closely related to other important coronavirus species and subspecies, so detection assays can give false-positive results if they are not efficiently specific to SARS-CoV-2. Thirdly, patients with suspected SARS-CoV-2 infection sometimes have a different respiratory viral infection or co-infections with SARS-CoV-2 and other respiratory viruses (MedRxiv 2020a;1-18). Confirmation of the COVID-19 is performed mainly by virus isolation followed by RT-PCR and sequencing (N. Engl J Med 2020;382:727-33, MedRxiv 2020a, Turkish J Biol 2020;44:192-202). The emergence and outbreak of the novel coronavirus highlighted the urgent need for new therapeutic technologies that are fast, precise, stable, easy to manufacture, and target-specific for surveillance and treatment. Molecular biology tools that include gene-editing approaches such as CRISPR-Cas12/13-based SHERLOCK, DETECTR, CARVER and PAC-MAN, antisense oligonucleotides, antisense peptide nucleic acids, ribozymes, aptamers, and RNAi silencing approaches produced with cutting-edge scientific advances compared to conventional diagnostic or treatment methods could be vital in COVID-19 and other future outbreaks. Thus, in this review, we will discuss potent the molecular biology approaches that can revolutionize diagnostic of viral infections and therapies to fight COVID-19 in a highly specific, stable, and efficient way. | en |
dc.identifier | 28 | |
dc.identifier.citation | Berber, B., Aydin, C., Kocabas, F., Guney-Esken, G., Yilancioglu, K., Karadag-Alpaslan, M., ... & Tastan, C. (2021). Gene editing and RNAi approaches for COVID-19 diagnostics and therapeutics. Gene therapy, 28(6), 290-305. | |
dc.identifier.issn | 0969-7128 | |
dc.identifier.pubmed | 33318646 | |
dc.identifier.scopus | 2-s2.0-85105424610 | |
dc.identifier.uri | https://doi.org/10.1038/s41434-020-00209-7 | |
dc.identifier.uri | https://hdl.handle.net/11413/7944 | |
dc.identifier.wos | 000598702600001 | |
dc.language.iso | en | |
dc.publisher | Springer Nature | |
dc.relation.journal | Gene Therapy | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | SARS-CoV-2 | |
dc.subject | Therapy | |
dc.subject | Ribozymes | |
dc.title | Gene Editing and RNAi Approaches for COVID-19 Diagnostics and Therapeutics | en |
dc.type | Review | |
dspace.entity.type | Publication | |
local.indexed.at | wos | |
local.indexed.at | pubmed | |
local.indexed.at | scopus | |
local.journal.endpage | 305 | |
local.journal.issue | 6 | |
local.journal.startpage | 290 |