Publication: Spatial arrangement of the animal male germ cell genome: I. Non-random pattern of radiation-induced inversions involving the vestigial region in autosome 2 of Drosophila melanogaster
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Alexandrov, Igor
Alexandrova, Margarita V.
Korablinova, Svetlana V.
Korovina, Larisa N.
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Abstract
At present, given a bulk of models for higher-order
chromatin structure in interphase nuclei of animal
somatic cells, little is known about the spatial
chromosome organization in animal male germ
cells mainly due to the lack suitable methods for
detailed observation and analysis without
disruption of the existing organization. We
pioneered in the study of this issue via analysis of
radiation-induced inversion patterns in Drosophila
male germ cell genome taking into account the fact
that the formation of inversion requires the spatial
proximity and contact of its ends. Analysis of 72 γ-
ray- or neutron-induced vg inversions in which the
first break is invariantly associated with the
vestigial (vg) gene in the middle of 2R autosome
shows the second inversion breakpoints that highly
non-randomly distributed over the entire second
chromosome clustering at the three autosome 2
“hot” chromosome areas. These findings show that
the polar Rabl-configuration of interphase
chromosomes in various somatic cells is not
typical for the haploid genome of Drosophila mature sperms. The specific megarosetteloopstructure
of haploid chromosome in the
mature animal sperms is proposed and justified.