Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Çukurovada Yetiştirilen Amsoy-71 ve Calland Soya Çeşitlerinde Verim ve Yağ Oranının Nükleer Teknikle Geliştirilmesi(TÜBİTAK Yayınları, 1986) Özbek, N.; Atilla, S.; ATAK, ÇİMEN; 6653Publication Comparison of Tolerance Related Proteomic Profiles of Two Drought Tolerant Tomato Mutants Improved by Gamma Radiation(Elsevier B.V., 2021) ÇELİK, ÖZGE; AYAN, ALP; MERİÇ, SİNAN; ATAK, ÇİMENLycopersicon esculentum L., also known as tomato, is an important industrial plant due to its products which worth billions of dollars annually, besides its nutritional value and health benefits. In this study, we investigated the two-dimensional protein expression profiles in drought tolerant mutant plants derived from industrial 5MX12956 tomato variety by Cs-137 gamma radiation source induced mutations. Drought tolerance of mutants were evaluated and confirmed by in vivo and in vitro methods. Eleven drought responsive protein spots were identified by two-dimensional electrophoresis and MALDI-TOF-MS. Identified proteins which presented differential expression under drought conditions were clustered under six distinct groups based on their cellular functions. These clusters are ATP and carbohydrate metabolism, mRNA processing and protein phosphorylation, oxidation reduction and stress response, signaling and supporting cytoskeleton. Our results contributed proteomic data to drought tolerance of our tomato mutants which were originated from drought susceptible 5MX12956 variety. They may also facilitate basis for future investigations into the genetic and physiological aspects of this tolerance. © 2021 Elsevier B.V.Publication Selection and Molecular Profiling Of Salt Tolerant Tobacco Plants Obtained By Gamma Radiation(2010) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Micropropagation of Anthurium adreanum from leaf explants(Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey, 2009) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Anthurium propagation by tissue culture techniques appears as an alternative to increase the production. Plant regeneration of Anthurium andraeanum cultivars “Arizona” and “Sumi” (Anthura) has been achieved through adventitious shoots formation from callus. The leaf explants were used for the propagation by indirect organogenesis. Callus induction medium consisted of half-strength MS basal salt with 0.6 mg/l 2,4-D, 1 mg/l BA. Shoot regeneration medium consisted of modified halfstrength MS salts with NH4NO3 lowered to 250 mg/l, 0.1 mg/l 2, 4-D and 1 mg/l BA. Rooting medium was (modified half-strength MS salts) supplemented with 1mg/l IBA and 0.04%active charcoals. The callus induction rate of Arizona variety was higher as compared to Sumi variety and this increase was found significant. The number of shoots per explants for Arizona variety also significantly increased as compared to Sumi variety. Arizona and Sumi root percentages were high and these percentages were 98% and 95% respectively. The results showed that Arizona variety had different responses as compared to Sumi variety for callus induction and the number of shoots per explants. Shoot multiplication for two varieties increased in the multiplication stages and this increase in number of shoots per explants were found important (p<0.05).Publication Effects of Salinity Stress on Densities of Trichomes, Glandular Trichomes, and Stomata in Two Soybean Varieties(Springer, 233 Spring St, New York, Ny 10013 USA, 2010-09) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 4019; 6653Publication Effect of magnetic field on peroxidase activities of soybean tissue culture(DIAGNOSIS PRESS LTD, 67 DONDUKOV BLVD, 1504 SOFIA, BULGARIA, 2007-05) Olgun, Atok; Alikamanoğlu, Sema; Rzakoulieva, A.; ATAK, ÇİMEN; ÇELİK, ÖZGE; TR6653; TR141172In this study, the aim was to determine the effect of magnetic field on peroxidase activities of soybean tissue culture. Shoot tips were put into petri dishes and exposed to a magnetic field for a period of 2.2 and 19.8 s at a magnetic flux of 2.9-4.6 mT. The shoot and root formation rate, fresh weights, chlorophyll quantities, total RNA concentrations and peroxidase activities of regenerated shoots from control and treated shoot tips were determined. While the rate of shoot formation was 28.57% in the control group, this rate was increased to 94.33% and 78.18%, respectively, in the explants that were exposed to a magnetic field for a period of 2.2 and 19.8 s. While the percentage of root formation in controls was 4.76%, this rate increased to 47.17% and 54.54%, respectively, in those that were exposed to a magnetic field at the same periods. When the fresh weights were determined, we found that the fresh weights of plantlets regenerated from treated explants were increased relative to controls. Chlorophyll a, chlorophyll b and total chlorophyll contents increased 21%, 13% and 18%, respectively, relative to control groups at 2.2 s. Peroxidase activity significantly increased in all magnetic field treatments (p<0.05). The total RNA concentration of seedlings regenerated from treatment explants significantly increased relative to controls (p<0.05). The regeneration and plant growth of shoot tips exposed to a magnetic field with a 2.2 s period were positively affected by the MF and increased with respect to controls and the length of time exposed.Publication Effect of magnetic field on Paulownia seeds(2000) Yurttaş, B.; Alikamanoğlu, S.; Topçul, F.; Rzakoulieva, A.; Danilov, V.; ATAK, ÇİMEN; 6653Publication Micropropagation of Anthurium spp(InTech Open Science, 2012) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Differential regulation of antioxidative gene expressions in response to salt stress in rice(International Congress on Transcriptomics 27-29 Temmuz 2015, 2015) Candar, Bilgin; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 195745; 6653Rice (Oryza sativa L.) is one of the most important crops because it is a nutritional source of more than one-third of the world population. Soil salinity is one of the most important abiotic stress factors that affect plant growth and productivity adversely. Rice growth and yield is also affected by salinity and at the seedling stage although it is known to be susceptible to salinity. Reactive oxygen species are induced by salt stress and some responsive mechanisms are evolved against to the detrimental effects caused by salt stress. Salinity response is especially controlled by obtaining homeostasis between antioxidative mechanisms and accumulation of reactive oxygen species (ROS) produced as a result of oxidative stress caused by salinity. It is known that combined expression profiles of antioxidative system enzymes may provide increased tolerance capacity. Therefore, the expression profiles of antioxidative enzymes in two different rice cultivars which have different salt sensitivities at four different salt stress conditions were determined. Salinity responses of two different rice varieties were investigated at seedling stage. Four different salinity treatments were then applied using Yoshida solution containing 0, 30, 90, 150 and 210 mM NaCl to the nutrient solution for seven days. At the end of the seven days, the leaves were harvested and stored at -20oC for further experiments. The salt stress responsive gene specific primers were amplified by designed primers due to the 3?-UTR regions of each of the following genes by aligning all available related genes in the databases of NCBI and KOME: Mn-SOD, Cu/Zn-SOD, Fe-SOD, Cytosolic APX, Thylakoid-bound APX, stromal APX, Cytosolic GR and CatA. Mn-SOD was consisted with the trend of variation in SOD activities of rice varieties. The expression patternof CAT A gene was markedly decreased compared to control in both rice varieties. Str-APX gene expression was up-regulated during salt stress treatments in both rice varieties. Transcript levels of Cyt-APX and Thy-APX were up-regulated in accordance with increasing salt stress in Osmanc?k-97 variety. The expression pattern of gene encoding enzyme Cyt-GR1 showed a gradual up-regulation as a response to subjected increasing NaCl stress in Mevl�tbey variety while only after 90 mM treatment, an up-regulation was observed for Osmanc?k variety. These data indicated that the antioxidative responses of salt tolerant and salt sensitive rice varieties are differentially regulated.Publication Magnetik alanın Soya Glycine Max L Merrill tohumları üzerine etkisi(1996) Yurttaş, B.; Yalçın, S.; Mutlu, D.; Azakoulieva, A.; ATAK, ÇİMEN; 6653