Moleküler Biyoloji ve Genetik Bölümü / Department of Molecular Biology and Genetics
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Browsing Moleküler Biyoloji ve Genetik Bölümü / Department of Molecular Biology and Genetics by Subject "accumulation"
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Publication Expression Analysis of Proline Metabolism-Related Genes in Salt-Tolerant Soybean Mutant Plants(Southern Cross Publ, 8 91-93 Mckenzie St, Lismore, Nsw 2480, Australia, 2013-09) Ünsal, Selin Gül; ÇELİK, ÖZGE; 113987Salt stress is one of the important abiotic stress factors. Proline is generally thought to play an important role in the improvement of salt tolerance in plants. In the present study, we discussed the relationship between free proline accumulation and the expression patterns of the genes that play roles in proline metabolism (P5CS, P5CR, PDH, P5CDH) under 90 mM NaCl stress. We used three salt tolerant M3 generation soybean mutant plants (Ataem-7/150-68, S04-05/150-2 and S04-05/150-114). The mutants belonging to M3 generation are determined as tolerant to 90 mM NaCl. The free proline contents of the salt-tolerant mutants were measured at the upper phase of the extract with respect to toluene. We observed 1.96-, 2.43- and 1.14-fold increases in the free proline accumulation of Ataem-7/150-68, S04-05/150-2 and S04-05/150-114 mutant plants after 7 days of salt treatment in accordance with control groups, respectively. The expression analyses were performed using specific primers designed for soybean gene regions. According to the results of the quantitative reverse-transcriptase polymerase chain reaction, all the genes were up-regulated when these mutants were subjected to salt stress. In addition to increased expression levels of these genes in three salt tolerant soybean mutants, the only statistically significant relation was observed between the regulation of P5CR and PDH gene expressions and proline content in S04-05/150-114 mutant. In further studies, the other possible mechanisms that cause proline accumulation should be evaluated for these salt tolerant soybean mutants.Publication The Effect Of Salt Stress On Antioxidative Enzymes And Proline Content Of Two Turkish Tobacco Varieties(Tubitak Scientific & Technical Research Council Turkey, Ataturk Bulvari No 221, Kavaklidere, Ankara, 00000, Turkey, 2012) ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987The aim of this study was to compare the salinity tolerances of 2 oriental tobacco varieties (Izmir Ozbas and Akhisar 97). Salinity stress experiments were performed under both in vitro and in vivo conditions. Seedlings of each variety were subjected to 0, 50, 100, 150, 200, 250, 300, and 350 mM NaCl. Photosynthetic pigment levels, lipid peroxidation rate, total protein content, antioxidant enzyme activities, and proline concentrations were determined for seedlings treated with salt for 14 days. The Akhisar 97 variety was found to be more sensitive to salinity stress than the Izmir Ozbas variety. Although proline is thought to accumulate in salt-tolerant plants, we found a negative correlation between salinity tolerance and proline accumulation in the plants. According to biochemical analyses, there were no differences in SOD, APX, GPX, or CAT activity levels between the 2 varieties, either in vivo or in vitro. However, differences in glutathione reductase (GR) activity between control plants and plants under NaCl stress were statistically significant in both varieties, both in vitro and in vivo. Our results support the hypothesis that GR is a key element in the evaluation of salinity tolerance of tobacco varieties.