TRDizin İndeksli Yayınlar / TRDizin Indexed Publications

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Browsing TRDizin İndeksli Yayınlar / TRDizin Indexed Publications by Rights "Attribution-NoDerivs 3.0 United States"

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    PublicationOpen Access
    The Comparison of Differentially Expressed microRNAs in Bag-1 Deficient and Wild Type MCF-7 Breast Cancer Cells by Small RNA Sequencing
    (TUBITAK Scientific & Technical Research Council Turkey, 2022) KILBAŞ, PELİN ÖZFİLİZ; Alkurt, Gizem; Çoker Gürkan, Ajda; DİNLER DOĞANAY, GİZEM; ARISAN, ELİF DAMLA; YERLİKAYA, PINAR OBAKAN
    The multifunctional BAG-1 (Bcl-2 athanogene-1) protein promotes breast cancer survival through direct or indirect interaction partners. The number of the interacting partners determines its cellular role in different conditions. As well as interaction partner variability, the amount of BAG-1 protein in the cells could cause dramatic alterations. According to previous studies, while the transient silencing of Bag-1 enhanced drug-induced apoptosis, deletion of BAG-1 could induce stemness properties and Akt-mediated actin remodeling in MCF-7 breast cancer cells. Considering the heterogeneity of breast cancer and the variability of BAG-1-mediated cell response, it has become essential to determine microRNA (miRNA) functions in breast cancer depending on Bag-1 expression level. This study aims to compare microRNA expression levels in wt and Bag-1 knockout (KO) MCF-7 breast cancer cells. hsa-miR-429 was selected as a potential miRNA in BAG-1KO MCF-7 cells because of the downregulation both in bioinformatics and validation qRT-PCR assay. According to predicted mRNA targets and functional enrichment analysis the ten hub proteins that are phosphatidylinositol4,5-biphosphate 3-kinase catalytic subunit alpha (PIK3CA), kinase insert domain receptor (KDR), GRB2 associated binding protein 1 (GAB1), Rac family small GTPase1 (RAC1), vascular endothelial growth factor A (VEGFA), Cbl proto-oncogene (CBL), syndecan 2 (SDC2), phospholipase C gamma 1 (PLCG1), E1A binding protein p300 (EP300), and CRK like proto-oncogene, adaptor protein (CRKL) were identified as targets of hsa-miR-429. The functional enrichment analysis showed that the most significant proteins were enriched in PI3K/Akt, focal adhesion, cytoskeleton regulation, proteoglycans in cancer, and Ras signaling pathways. It was determined that hsa-miR-429 targeted these pathways in Bag-1 deficient conditions and could be used as a potential therapeutic target in future studies.
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    PublicationRestricted
    Minimal Generators of Annihilators of Even Neat Elements in the Exterior Algebra
    (Scientific Technical Research Council Turkey-TUBITAK, 2022) ESİN, SONGÜL
    This paper deals with an exterior algebra of a vector space whose base field is of positive characteristic. In this work, a minimal set of generators forming the annihilator of even neat elements of such an exterior algebra is exhibited. The annihilator of some special type of even neat elements is determined to prove the conjecture established in [3]. Moreover, a vector space basis for the annihilators under consideration is calculated.
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    PublicationOpen Access
    The Protective Impact of Growth Hormone Against Rotenone-Induced Apoptotic Cell Death via Acting on Endoplasmic Reticulum Stress and Autophagy Axis
    (Scientific and Technological Research Council Turkey, 2023) RENCÜZOĞULLARI, ÖZGE; TORNACI, SELAY; ÇELİK, YAĞMUR; TAŞ, NAYAT NAROT; YERLİKAYA, PINAR OBAKAN; Arısan, Elif Damla; Gürkan, Ajda Çoker
    Human growth hormone (GH) is crucial modulator of cellular metabolisms, including cell proliferation and organ development, by stimulating insulin-like growth factor-1 (IGF-1), which has various functions such as cell proliferation, tissue growth, survival, or neuroprotection. Therefore, GH is implicated as a critical player in the cell and can enhance neurogenesis and provide neuroprotection during the treatment of neurological diseases such as Parkinson's disease (PD). In this study, the neuroprotective role of GH was investigated in rotenone-induced PD models for the first time. Both SH-SY5Y and SK-N-AS neuroblastoma cells were exposed to rotenone to mimic PD pathogenesis as stated in previous studies. Our data demonstrated that overexpression of GH led to the resistance of the SH-SY5Y and SK-N-AS cell lines to rotenone treatment. The levels of ER stress markers, CHOP, PERK, XBP-1, and ATF6, were higher in wt cells than GH+ SH-SY5Y cells. However, the level of autophagy markers LC3 increased and the levels of reactive oxygen species (ROS) decreased with the overexpression of GH. Furthermore, while rotenone significantly increased the SubG1 population in the cell cycle of SH-SY5Y wt cells, there was a minor alteration in GH+ cell population. Concomitantly, the levels of the proapoptotic marker, cleaved-PARP, and positive staining of Annexin V in SH-SY5Y wt cells were higher after rotenone treatment. Together, these results revealed that overexpression of GH enhanced the autophagy response by triggering the ER stress of SH-SY5Y cells to rotenone exposure and showed a neuroprotective effect in vitro PD models.
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    PublicationOpen Access
    Wearable Sensor Device for Posture Monitoring and Analysis During Daily Activities: A Preliminary Study
    (Dr. Ceyhun YILMAZ, 2022) AKBULUT, FATMA PATLAR; ÖZGÜL, GİZEM
    The increase in technological advancements in recent years has led to the emergence of a new lifestyle. Although being assisted by machines for small-scale tasks in daily housework makes daily life easier, this has caused people to reduce their daily active movements and negatively affects human health. Especially during the COVID-19 pandemic, with the conversion of the working style to the home environment, working hours spent at the desk are more than ever. Due to the prolongation of the working time, the employees stay in the same position more inactive, thus their muscles weaken and they start to have muscle disease. Weaknesses in the muscles have occurred to the formation of postural problems in people. In our study, a smart vest system was developed to detect and control posture disorders. The proposed system is designed to recommend the most suitable exercises to avoid any physical discomforts. It is also aimed to detect hunched posture by collecting data on the person wearing the vest through sensors. Besides, it is encouraged to correct the posture disorder by warning the person audibly during the hunched posture. The experiments conducted with eight participants showed that the proposed system warns the users with necessary posture corrections, proving its potential use.