Advances in Molecular Biology / Moleküler Biyolojide Gelişmeler

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Browsing Advances in Molecular Biology / Moleküler Biyolojide Gelişmeler by Author "Açık, Leyla"

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    PublicationOpen Access
    Genetic differentiation of Turkish Althaea L. and Alcea L. species
    (İstanbul Kültür Üniversitesi, 2009) Öztürk, Fatma; Babaoğlu, Selcen; Uzunhisarcıklı, M. Erkan; Açık, Leyla; Vural, Mecit; Gürcan, I. Sefa
    Random amplified polymorphic DNA (RAPD) analysis and SDS-PAGE analysis of total seed proteins were used to estimate genetic differences between/within four Althaea (Malvaceae) and eighteen Alcea species. The 4 Althaea and 18 Alcea species were found to have species-specific protein profiles. The total of 223 different RAPD bands for Althaea species were generated by the 11 primers and total of 116 different RAPD bands by the 8 random primers for Alcea species. The results of the POPGENE cluster analysis were in broad agreement with previous morphological classifications of these four species. Statistical significance within Alcea and Althaea population was tested using of molecular variance (AMOVA) approach used in Arlequin. According to the results, there is a large genetic differentiation between species A. hirsuta with A. armeniaca, A. officinalis with A. hirsuta, FST 0.24 and FST 0.21, respectively. There is also wide differentiation between Alcea species. FST statistics range from 0,126 (A. guestii between A. striata ssp. striata) to 0,685 (A. biennis between A. acaulis). The results suggest that RAPD and SDS-PAGE markers can be used to reveal genetic differentiation between species and genus.
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    PublicationOpen Access
    Identification of clinic uropathogen Escherichia coli isolates by antibiotic susceptibility, plasmid and whole cell protein profiles
    (İstanbul Kültür Üniversitesi, 2007-03) Çelebi, Ayten; Duran, Nizam; Öztürk, Fatma; Açık, Leyla; Aslan, Gönül; Aslantaş, Özkan
    The aim of this research was to evaluate the protein, plasmid and antibiotic resistance patterns among 118 uropathogen E. coli strains from infected urinary systems. Plasmids were detected 113 strains (97%). Some isolates harboured up to 10 plasmids, ranging from 1 to 19 kb in size. The total whole cell protein profiles of the strains were obtained using the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) method. The protein bands were stained with Coomassie-blue and analyzed by statistics package POPGEN. The 118 E. coli were also analyzed for their resistance to antimicrobial agents. The highest rates of resistance were against ampicillin (61 %) and amoxicillin-clavulanic acid (46.6 %). The most common antimicrobial resistance of these isolates was ampicillin, amoxicillin-clavulanic acid, trimethoprimsulfamethoxazole, gentamicin, ciprofloxacin, amikacin, cefoxitin, and ceftriaxone. Multiple resistance to all antibiotics except imipenem was observed in 5 isolates. Similarity matrix and dendrograms were generated by using UPGMA algorithm which made it possible to evaluate the similarity or intra-specific polymorphism degrees based on whole-cell protein fingerprinting, plasmid profiles and antibiotic resistance pattern. It was determined that the SDS-PAGE method may provide better criteria than plasmid and antimicrobial susceptibility for the taxonomic and epidemiological studies of E. coli isolates.
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    PublicationOpen Access
    Ras oncogenes polymorphism in Turkish thyroid cancer patients
    (İstanbul Kültür Üniversitesi, 2008) Öztürk, Fatma; Daloğlu, Cihan; Açık, Leyla; Kılıç, Mehmet; Koç, Mahmut
    Molecula r mutations to proto - oncogene sequences may be involved in the pathogenesis of human thyroid neoplasm. Problems on oncogenes and tumor supressor genes activation in cell circle could cause tumor. Many oncogenes and tumor suppressing genes exist in varying percentages in various types of thyroid cancers. Ras, Gsp, Ret or Trk oncogenes can be involve in thyroid tumors . Members of t h e Ras gene family (H-ras, K-ras, and N-ras) are signal transferring proteins. These genes codes for 21 kDa GTP binding proteins. We studied 24 thyroid cancer and 77 control for ras gene point mutations in two different codons (12 and 13) using a restriction fragment length polymorphism technique. According to enzyme digesting, no c-K-ras gene codon 12/13 and N-ras gene codon 12 point mutation were observed in any of the samples we studied .