RAPD Analysis of Salinity and Drought Tolerant Soybean (Glycine max (L.) Merr) Mutants Generated by Gamma Radiation

Abstract

Aim of this study is to evaluate genetic distances between M4 generation salinity and drought tolerant soybean mutants by using RAPD primers. AM1, SM1, SM2, SM3 and SM5 mutants were generated from present Ataem-07 and S04-05 soybean varieties by using Cs-137 gamma radiation source. All of the mutants are determined to be 90 mM NaCI and 5 days of drought tolerant comparing to their respected original varieties. Jaccard genetic similarity matrixes of mutants and their original varieties were generated according to the band profiles under untreated, drought and salt treated conditions. UPGMA dendograms were drawn based on the genetic similarity coefficients. In salt treated experimental groups, 22 random RAPD primers presented 61 polymorphic band among 191 total bands. Polymorphism ratio was calculated as 31.12 %. In drought treated experimental groups, 21 random RAPD primers were used and 68 in total of 203 bands were found to be polymorphic. Polymorphism ratio was calculated as 33.49%. Both salt and drought treated SM1 and SM3 mutants were found to be distant from their untreated controls.