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Composition analysis of soybean salt resistant mutants by raman spectroscopy and chemometrics

dc.contributor.authorYılmaz, Ayberk
dc.contributor.authorKabuk, Hayrunnisa Nur
dc.contributor.authorKaygısız, Ersin
dc.contributor.authorFausto, Rui
dc.contributor.authorMERİÇ, SİNAN
dc.contributor.authorAYAN, ALP
dc.contributor.authorATAK, ÇİMEN
dc.contributor.authorÇELİK, ÖZGE
dc.contributor.authorILDIZ, GÜLCE ÖĞRÜÇ
dc.contributor.authorID107326tr_TR
dc.contributor.authorID6653tr_TR
dc.contributor.authorID175748tr_TR
dc.contributor.authorID185510tr_TR
dc.contributor.authorID219257tr_TR
dc.contributor.authorID113987tr_TR
dc.date.accessioned2019-09-05T08:58:31Z
dc.date.available2019-09-05T08:58:31Z
dc.date.issued2019
dc.description.abstractSoya seeds belonging to fourth generation mutants (M<i) of two different salt-tolerant soybeans (Alaem-7 cultivar (A7) variety and S04-05 (S) breeding line) together with the corresponding controls were studied by Raman spectroscopy, complemented by chemometrics methods, and compared with the data obtained by the ultraviolet-visible-near infrared (UV-Vis- NIR) reflectance spectroscopy standard method. It is shown that gamma irradiation caused significant changes in the lipid-to-protein ratio of the studied mutants of the two soybean varieties, compared to the corresponding control group. An increase in the lipid-to-protein ratio was observed in the case of the studied Ataem-7 variety mutants, while in the investigated S04- 05 breeding line mutants this ratio was found to reduce. The obtained results show that gamma irradiation can be offered as technique to enrich the mutant soybeans either in proteins or lipids, according to their desired application. Also, the observed increase in the intensity of the characteristic Raman bands of phenylalanine of S-mutants, compared to those appearing in the Raman spectra of the control samples, indicates an increase in the relative amount of this nutritionally relevant amino acid in the genetically modified plants’ seeds.Both Ward’s cluster analysis and principal component analysis (PCA) showed a clear discrimination between mutants and controls (see Figure), which is in accordance with the compositional information extracted from the Raman bands’ intensity analysis and reflectance spectroscopy measurements.tr_TR
dc.identifier.urihttps://hdl.handle.net/11413/5241
dc.language.isoen_UStr_TR
dc.relation.journalAnalytical and Bionalytical Methods Conferencetr_TR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.titleComposition analysis of soybean salt resistant mutants by raman spectroscopy and chemometricstr_TR
dc.typeconferenceObjecttr_TR
dspace.entity.typePublication
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relation.isAuthorOfPublicationef7690fd-a4d2-4926-bd2c-fc64ea6f7542
relation.isAuthorOfPublication.latestForDiscovery45f4e864-bc25-44f1-bd85-d90af16e4cc6

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