RENCÜZOĞULLARI, ÖZGEFreitas, Ines LuaRadzali, SyanasKeskin, BuseKothari, ArchanaWarford, AntonyUysal-Onganer, PınarARISAN, ELİF DAMLA2022-11-182022-11-182020Arisan, E. D., Rencuzogullari, O., Freitas, I. L., Radzali, S., Keskin, B., Kothari, A., ... & Uysal-Onganer, P. (2020). Upregulated Wnt-11 and miR-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells. Biology, 9(3), 52.https://doi.org/10.3390/biology9030052https://hdl.handle.net/11413/7945Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, is commonly highly upregulated in many cancers, including PCa, and showed correlation with the Wnt-signaling axis to increase invasion. Wnt-11 is a developmentally regulated gene and has been found to be upregulated in PCa, but its mechanism is unknown. The present study aimed to investigate the roles of miR-21 and Wnt-11 in PCa in vivo and in vitro. First, different Gleason score PCa tissue samples were used; both miR-21 and Wnt-11 expressions correlate with high Gleason scores in PCa patient tissues. This data then was confirmed with formalin-fixed paraffin cell blocks using PCa cell lines LNCaP and PC3. Cell survival and colony formation studies proved that miR-21 involves in cells' behaviors, as well as the epithelial-mesenchymal transition. Consistent with the previous data, silencing miR-21 led to significant inhibition of cellular invasiveness. Overall, these results suggest that miR-21 plays a significant role related to Wnt-11 in the pathophysiology of PCa.eninfo:eu-repo/semantics/openAccessmicroRNAIn Situ HybridizationProstate CancerWnt-11miR-21Article0005251461000082-s2.0-85082061861321828392079-7737