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dc.contributor.authorÖzfiliz Kılbaş, Pelin
dc.contributor.authorAkçay, İzzet Mehmet
dc.contributor.authorDinler Doğanay, Gizem
dc.contributor.authorArısan, Elif Damla
dc.date.accessioned2019-06-19T06:36:13Z
dc.date.available2019-06-19T06:36:13Z
dc.date.issued2019-01-19
dc.identifier46tr_TR
dc.identifier46tr_TR
dc.identifier46tr_TR
dc.identifier.issn0301-4851
dc.identifier.urihttps://hdl.handle.net/11413/4873
dc.description.abstractThe multifunctional anti-apoptotic Bag-1 protein has important roles in apoptosis, proteasome-mediated degradation, transcriptional regulation, and intracellular signaling. Bag-1 promotes cell survival and proliferation, and is overexpressed in breast cancer. Therefore, Bag-1-targeted therapy might be a promising strategy to treat breast cancer. However, the effects of Bag-1 silencing in combination with conventional chemotherapeutic drugs on cell viability and major signaling pathways have not yet been fully investigated in breast cancer cells. In this study, we investigated the cytotoxic effects of Bag-1 silencing, alone and in combination with cisplatin or paclitaxel treatment, in MCF-7 breast cancer cells. Bag-1 knockdown by shRNA or siRNA transfection sensitized MCF-7 cells to apoptosis induced by cisplatin or paclitaxel. Combination of Bag-1 silencing and drug treatment more potently downregulated the pro-survival PI3K/Akt/mTOR and p44/42 mitogen activated protein kinase (MAPK) pathways, and more potently upregulated the stress-activated p38 and SAPK/JNK MAPK pathways. Bag1-silenced drug-treated cells had also highly reduced proliferative capacity, downregulated cyclin–cyclin dependent kinase complexes and upregulated tumor suppressors p21 and Rb. These results overall indicated that Bag-1 silencing enhanced cisplatin- or paclitaxel-induced cytotoxicity through multiple pathways. In conclusion, Bag-1 targeted therapy might enhance the therapeutic potential of conventional anti-cancer drugs in the treatment of breast cancer.tr_TR
dc.language.isoen_UStr_TR
dc.relationMolecular Biology Reportstr_TR
dc.subjectBag-1 siRNA/shRNAtr_TR
dc.subjectChemotherapeutic Agentstr_TR
dc.subjectApoptosistr_TR
dc.subjectBreast Cancertr_TR
dc.subjectMAPK · PI3K/Akt/mTOR Pathwaytr_TR
dc.titleBag-1 Silencing Enhanced Chemotherapeutic Drug-induced Apoptosis in MCF-7 Breast Cancer Cells Affecting PI3K/Akt/mTOR and MAPK Signaling Pathwaystr_TR
dc.typeArticletr_TR
dc.contributor.authorID195744tr_TR
dc.contributor.authorID152975tr_TR
dc.contributor.authorID113920tr_TR
dc.identifier.wos462022300084
dc.identifier.wos462022300084en
dc.identifier.scopus2-s2.0-85060347652
dc.identifier.scopus2-s2.0-85060347652en
dc.identifier.pubmed30661182
dc.identifier.pubmed30661182en


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