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dc.contributorFen Edebiyat Fakültesi / Faculty of Letters and Sciences Moleküler Biyoloji ve Genetik / Molecular Biology and Geneticstr_TR
dc.contributor.authorAtak, Çimen
dc.contributor.authorÇelik, Özge
dc.contributor.authorSuludere, Zekiye
dc.date.accessioned2018-10-19T14:45:19Z
dc.date.available2018-10-19T14:45:19Z
dc.date.issued2014
dc.identifier.issn1836-3644
dc.identifier.urihttp://dx.doi.org/10.4172/2155-952X.S1.027
dc.identifier.urihttps://hdl.handle.net/11413/2844
dc.description.abstractThis is the first report on soybean with the aim to show the effects of gamma radiation on trichome metabolim. Soybean seeds were subjected to 300 Gy gamma radiation at a dose rate of 10 Gy/min using a Cs-137 gamma source. The photosynthetic pigment, total protein content and ascorbate peroxidase activity were studied. The results showed that the chlorophyll a content was decreased by 80% on day 14 and by 77% on day 21 of irradiation. The chlorophyll b content was reduced by 58.6% and 62.06% on day 14 and 21 after irradiation, respectively. The total carotenoid concentration was reduced by 81.14% on the 14th day after irradiation and by 91% on the 21st day of irradiation, compared to control. The total protein concentration was found to have decreased significantly at 14 and 21 day after treatment. High level of ascorbate peroxidase (APX) activity was recorded in the leaves developed from irradiated soybean seeds, compared to the non-irradiated group. The trichome densities were 6.76 fold increased at 21 day of irradiation, while the stomatal densities were decreased, compared to control. We also performed a qRT-PCR analysis to detect the transcription levels of the soybean trichome developmental genes. The GL2 and CPC genes were up-regulated (P≤0.05). The results of this study pointed out that the CPC transcription factor has to be study in further studies to provide an insight on its exact role in regulation of trichome development in soybean under radiation stress.tr_TR
dc.language.isoen_UStr_TR
dc.relationPlant Omicstr_TR
dc.subjectGamma radiationtr_TR
dc.subjectGlycine maxtr_TR
dc.subjecttrichometr_TR
dc.subjectortholog genestr_TR
dc.subjectqRT-PCRtr_TR
dc.titleResponse of soybean plants to gamma radiation: Biochemical analyses and expression patterns of trichome developmenttr_TR
dc.typeArticletr_TR
dc.contributor.authorID6653tr_TR
dc.contributor.authorID113987tr_TR
dc.contributor.authorID4019tr_TR
dc.identifier.scopus2-s2.0-84912109790


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