• Home
  • About
  • Policies
  • Contact
    • Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
Advanced Search
View Item 
  •   Home
  • Fen Edebiyat Fakültesi / Faculty of Letters and Sciences
  • Moleküler Biyoloji ve Genetik / Molecular Biology and Genetics
  • Makaleler / Articles
  • View Item
  •   Home
  • Fen Edebiyat Fakültesi / Faculty of Letters and Sciences
  • Moleküler Biyoloji ve Genetik / Molecular Biology and Genetics
  • Makaleler / Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Inhibition of autophagy enhances DENSpm-induced apoptosis in human colon cancer cells in a p53 independent manner

Thumbnail
Author
Çoker Gürkan, Ajda
Arısan, Elif Damla
Obakan Yerlikaya, Pınar
İlhan, Halime
Ünsal Palavan, Zeynep Narçın
Type
Article
Date
2018-06
Language
en_US
Metadata
Show full item record
Abstract
One of the recently developed polyamine (PA) analogues, N (1) ,N (11)-diethylnorspermine (DENSpm), has been found to act as an apoptotic inducer in melanoma, breast, prostate and colon cancer cells. Also, its potential to induce autophagy has been established. Unfolded protein responses and starvation of amino acids are known to trigger autophagy. As yet, however, the molecular mechanism underlying PA deficiency-induced autophagy is not fully clarified. Here, we aimed to determine the apoptotic effect of DENSpm after autophagy inhibition by 3-methyladenine (3-MA) or siRNA-mediated Beclin-1 silencing in colon cancer cells. The apoptotic effects of DENSpm after 3-MA treatment or Beclin-1 silencing were determined by PI and AnnexinV/PI staining in conjunction with flow cytometry. Intracellular PA levels were measured by HPLC, whereas autophagy and the expression profiles of PA key players were determined in HCT116, SW480 and HT29 colon cancer cells by Western blotting. We found that DENSpm-induced autophagy was inhibited by 3-MA treatment and Beclin-1 silencing, and that apoptotic cell death was increased by PA depletion and spermidine/spermine N (1)-acetyltransferase (SSAT) upregulation. We also found that autophagy inhibition led to DENSpm-induced apoptosis through Atg5 down-regulation, p62 degradation and LC3 lipidation in both HCT116 and SW480 cells. p53 deficiency did not alter the response of the colon cancer cells to DENSpm-induced apoptotic cell death under autophagy suppression conditions. From our results we conclude that DENSpm-induced apoptotic cell death is increased when autophagy is inhibited by 3-MA or Beclin-1 siRNA through PA depletion and PA catabolic activation in colon cancer cells, regardless p53 mutation status.
Subject
Colon cancer
Apoptosis
Autophagy
Polyamine analogue
DENSpm
Human-Melanoma Cells
Polyamine Analog N-1,N-11-Diethylnorspermine
Spermidine/Spermine N-1-Acetyltransferase
Carcinoma-Cells
Induction
Resistance
Death
Metabolism
Catabolism
Modulators
URI
https://doi.org/10.1007/s13402-017-0369-x
https://hdl.handle.net/11413/2294
Collections
  • Makaleler / Articles [140]
  • Pubmed Publications [149]
  • Scopus Publications [724]
  • WoS Publications [1016]

İstanbul Kültür University

Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS

İKU Kütüphane


Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeLanguageBy PublisherRightsPubmedScopusWoSThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeLanguageBy PublisherRightsPubmedScopusWoS

My Account

Login

İstanbul Kültür University

Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS