Show simple item record

dc.contributor.authorÇelebi, Ayten
dc.contributor.authorDuran, Nizam
dc.contributor.authorÖztürk, Fatma
dc.contributor.authorAçık, Leyla
dc.contributor.authorAslan, Gönül
dc.contributor.authorAslantaş, Özkan
dc.date.accessioned2014-07-09T09:59:09Z
dc.date.available2014-07-09T09:59:09Z
dc.date.issued2007-03
dc.identifier.issn1307-3923
dc.identifier.urihttp://hdl.handle.net/11413/169
dc.description.abstractThe aim of this research was to evaluate the protein, plasmid and antibiotic resistance patterns among 118 uropathogen E. coli strains from infected urinary systems. Plasmids were detected 113 strains (97%). Some isolates harboured up to 10 plasmids, ranging from 1 to 19 kb in size. The total whole cell protein profiles of the strains were obtained using the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) method. The protein bands were stained with Coomassie-blue and analyzed by statistics package POPGEN. The 118 E. coli were also analyzed for their resistance to antimicrobial agents. The highest rates of resistance were against ampicillin (61 %) and amoxicillin-clavulanic acid (46.6 %). The most common antimicrobial resistance of these isolates was ampicillin, amoxicillin-clavulanic acid, trimethoprimsulfamethoxazole, gentamicin, ciprofloxacin, amikacin, cefoxitin, and ceftriaxone. Multiple resistance to all antibiotics except imipenem was observed in 5 isolates. Similarity matrix and dendrograms were generated by using UPGMA algorithm which made it possible to evaluate the similarity or intra-specific polymorphism degrees based on whole-cell protein fingerprinting, plasmid profiles and antibiotic resistance pattern. It was determined that the SDS-PAGE method may provide better criteria than plasmid and antimicrobial susceptibility for the taxonomic and epidemiological studies of E. coli isolates.tr_TR
dc.language.isoen_UStr_TR
dc.publisherİstanbul Kültür Üniversitesitr_TR
dc.subjectEscherichia colitr_TR
dc.subjecturinary systemtr_TR
dc.subjectantibiotic resistancetr_TR
dc.subjectplasmid profiletr_TR
dc.subjectSDS-Pagetr_TR
dc.subjectüriner sistemtr_TR
dc.subjectplazmid profilitr_TR
dc.subjectantibiyotik direncitr_TR
dc.titleIdentification of clinic uropathogen Escherichia coli isolates by antibiotic susceptibility, plasmid and whole cell protein profilestr_TR
dc.typeArticletr_TR


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record