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dc.contributor.authorObakan Yerlikaya, Pınar
dc.contributor.authorArısan, Elif Damla
dc.contributor.authorÇoker Gürkan, Ajda
dc.contributor.authorAdacan, Kaan
dc.contributor.authorÖzbey, Utku
dc.contributor.authorSomuncu, Berna
dc.contributor.authorBaran, Didem
dc.contributor.authorÜnsal Palavan, Zeynep Narçın
dc.date.accessioned2018-07-23T08:29:25Z
dc.date.available2018-07-23T08:29:25Z
dc.date.issued2017-06
dc.identifier.issn0899-1987
dc.identifier.other1098-2744
dc.identifier.urihttps://doi.org/10.1002/mc.22616
dc.identifier.urihttps://hdl.handle.net/11413/2256
dc.description.abstractEpibrassinolide (EBR), a member of brassinostreoids plant hormones with cell proliferation promoting role in plants, is a natural polyhydroxysteroid with structural similarity to steroid hormones of vertebrates. EBR has antiproliferative and apoptosis-inducing effect in various cancer cells. Although EBR has been shown to affect survival and mitochondria-mediated apoptosis pathways in a p53-independent manner, the exact molecular targets of EBR are still under investigation. Our recent SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) data showed that the most significantly altered protein after EBR treatment was calreticulin (CALR). CALR, a chaperone localized in endoplasmic reticulum (ER) lumen, plays role in protein folding and buffering Ca2+ ions. The alteration of CALR may cause ER stress and unfolded protein response correspondingly the induction of apoptosis. Unfolded proteins are conducted to 26S proteasomal degradation following ubiquitination. Our study revealed that EBR treatment caused ER stress and UPR by altering CALR expression causing caspase-dependent apoptosis in HCT 116, HT29, DLD-1, and SW480 colon cancer cells. Furthermore, 48 h EBR treatment did not caused UPR in Fetal Human Colon cells (FHC) and Mouse Embryonic Fibroblast cells (MEF). In addition our findings showed that HCT 116 colon cancer cells lacking Bax and Puma expression still undergo UPR and related apoptosis. CALR silencing and rapamycin co-treatment prevented EBR-induced UPR and apoptosis, whereas 26S proteasome inhibition further increased the effect of EBR in colon cancer cells. All these findings showed that EBR is an ER stress and apoptotic inducer in colon cancer cells without affecting nonmalignant cells.tr_TR
dc.language.isoen_UStr_TR
dc.publisherWiley, 111 River St, Hoboken 07030-5774, NJ USAtr_TR
dc.relationMolecular Carcinogenesistr_TR
dc.subjectcolon cancertr_TR
dc.subjectendoplasmic reticulum stresstr_TR
dc.subjectepibrassinolidetr_TR
dc.subjectunfolded protein responsetr_TR
dc.subjectUnfolded Protein Responsetr_TR
dc.subjectEr Stresstr_TR
dc.subjectExpressiontr_TR
dc.subjectCalciumtr_TR
dc.subjectDeathtr_TR
dc.subjectPathwaytr_TR
dc.subjectDegradationtr_TR
dc.subjectMechanismstr_TR
dc.subjectCaspase-12tr_TR
dc.subjectChaperonestr_TR
dc.titleCalreticulin is a fine tuning molecule in epibrassinolide-induced apoptosis through activating endoplasmic reticulum stress in colon cancer cellstr_TR
dc.typeArticletr_TR
dc.contributor.authorID156421tr_TR
dc.contributor.authorID113920tr_TR
dc.contributor.authorID125860tr_TR
dc.contributor.authorID6125tr_TR


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