Fen Edebiyat Fakültesi / Faculty of Letters and Sciences

Permanent URI for this communityhttps://hdl.handle.net/11413/10

Browse

Browsing Fen Edebiyat Fakültesi / Faculty of Letters and Sciences by Author "Açık, Leyla"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • No Thumbnail Available
    PublicationMetadata only
    Genetic Analysis Of Rhododendron Mutants Using Random Amplified Polymorphic DNA (RAPD)
    (Pakistan Botanical Soc, Dept Of Botany Univ Karachi, 32 Karachi, Pakistan, 2011-04) Açık, Leyla; ATAK, ÇİMEN; ÇELİK, ÖZGE; TR6653; TR113987; TR1781
    The aim of our study was to consider the efficiency of randomly amplified polymorphic DNA (RAPD) analysis to show the differences between Rhododendron mutants and control plants. The mutant plantlets were selected due to their better In vitro shoot and root regeneration relative to control in both varieties. Six mutant plantlets from Alfred and seven mutant plantlets from Paars variety were selected from M(1)V(2) generations. These mutants were selected from plantlets which were irradiated with doses 5 and 10 Gy. They were grown up In vitro conditions. The dendrogram shows one big cluster and the others to be distinguished. Genetic variability induced with gamma ray and RAPD methods were used to detect mutations at the Rhododendron shoot culture. Our results show that Rhododendron mutants were district from controls.
  • No Thumbnail Available
    PublicationMetadata only
    Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD
    (2004-11-22) Alikamanoğlu, Sema; Açık, Leyla; Canpolat, Yasemin; ATAK, ÇİMEN; 6653; 176641; 1781
    The aim of our study was to induce with radiation of atrazine resistant and tolerated mutants in Coles, Amsoy-71 and 1937 soybean varieties. Atrazine that is photosynthetic inhibitor is the most important herbicide of S-triazin group, and shows toxic effect on soybean plant. For the improvement of the atrazine resistant plants with mutation breeding, the seeds belonging to the three varieties were irradiated with 200 Gy of gamma radiation dose. The irradiated seeds were sown in the field and at the end of harvesting season, every pod at node situated on the main stem was picked up separately and M2 generations were obtained. At the plants, which were obtained from M2 generation, chlorophyll mutants were determined and atrazine selection was made. The percentage of chlorophyll mutants for Amsoy-71, Coles and 1937 soybean varieties were found as 1.07, 1.48 and 1.32, respectively. At the end of atrazine selection, the percentages of atrazine resistant plants for Amsoy-71, Coles and 1937 soybean varieties were 0.80, 0.60 and 0.53, respectively. The percentages of atrazine tolerated plants were 1.07, 1.18 and 1.05, respectively as well. In our research; the differences among the mutants replying to atrazine in various concentrations were examined by using RAPD procedure as the molecular marker techniques in comparison with polymorphism. In the study done by using 14 primers; according to the amplification results, the differences between atrazine resistant plants were shown.